NIRx Newsletter Issue #1

 

Contents

NIRx End-User Spotlight

Prof. Niels Birbaumer’s team from The University of Tübingen receives global recognition for ALS research using fNIRS.


Recent Publications

See some of the latest publications using NIRx systems.


Software Release: NIRStar 15-0

Learn about the release of NIRx’s system control software, NIRStar.


NIRx’s New Dedicated Support Website

Learn about this great new resource and how our customers can register.


fNIRS Detectors: SiPD vs. APD

Get an overview of fNIRS detectors, including the difference between SiPDs and APDs.


Laser and LED Sources from NIRx

One of our most-asked questions is regarding the difference between Laser and LED sources, and why we offer both. Well, you can read about it here to find out.


NIRx End-User Spotlight

Prof. Niels Birbaumer of The University of Tübingen Receives Recognition for fNIRS/EEG BCI Research with ALS Patients

NIRx is extremely proud for being part of the groundbreaking research of the team of Prof. Niels Birbaumer, from the University of Tübingen (Germany). The work published in the journal of PLUS Biology [1] marks 25 years of their continuous work with completely locked-in ALS patients. The group has been successful in using fNIRS to open a communication channel with patients who have no other means of interacting with the external world. Patients were able to learn to modulate their cortical activity in order to answer to yes/no questions with an incredible 70% correct response rate.

Pictured right, From [1]: “Fig 2. Classification accuracy of Patient F. Linear SVM CA across “training sessions—offline CA” (histogram in grey),
“feedback sessions—online CA” (green dot), and “open question session—online CA” (plus sign in red), obtained using (A) relative change in O2Hb, (B) EEG, and (C) EOG data. The classification accuracy reported here is daywise, as all the “training sessions” in a day were used to calculate the average classification accuracy of all the “training sessions” in a day. In the figure panels A, B, and C, the x-axis is the number of days and the y-axis is the classification accuracy. The solid black and dotted horizontal lines represent the chance-level threshold calculated using the metric described in the BCI effectiveness metric section for “training sessions” and “feedback sessions,” respectively. Since the feedback during the feedback and open question sessions was provided using the O2Hb, the online CA of the feedback and open question sessions is reported only for the fNIRS data.
[1] "Brain–Computer Interface–Based Communication in the Completely Locked-In State.” Chaudhary et al. PLoS Biol 15 (1), e1002593. 2017 Jan 31.

NIRx has always firmly believed in the enormous potential of fNIRS but it is extremely heartwarming and encouraging to see that our technology truly helps improving people’s lives. The work of the group of Prof. Birbaumer has caught the attention of worldwide media and their results have been presented on CNN, BBC, The Guardian and Der Spiegel among others. Prof. Birbaumer, Dr. Ujwal Chaudhary (lead researcher) and their team are happy to answer questions related to their work on their Reddit thread.
 

By: Lamija Pašalić
Support Director

 

Recent Publications with NIRx Systems

Below are some of the latest Peer-Reviewed publications using NIRx NIRS systems. 
 

  • A. Galderisi et al., “Long-term continuous monitoring of the preterm brain with diffuse optical tomography and electroencephalography: a technical note on cap manufacturing,” Neurophoton, vol. 3, no. 4, pp. 045009–045009, 2016.
     
  • J. Shin et al., “Open Access Dataset for EEG+NIRS Single-Trial Classification,” IEEE Transactions on Neural Systems and Rehabilitation Engineering, vol. PP, no. 99, pp. 1–1, 2016.
     
  • L. Pollonini, H. Bortfeld, and J. S. Oghalai, “PHOEBE: a method for real time mapping of optodes-scalp coupling in functional near-infrared spectroscopy,” Biomed. Opt. Express, BOE, vol. 7, no. 12, pp. 5104–5119, Dec. 2016.
     
  • M. Balconi and M. E. Vanutelli, “Interbrains cooperation: Hyperscanning and self-perception in joint actions,” Journal of Clinical and Experimental Neuropsychology, vol. 0, no. 0, pp. 1–14, Nov. 2016.
     
  • J. Shin, K.-R. Müller, and H.-J. Hwang, “Near-infrared spectroscopy (NIRS)-based eyes-closed brain-computer interface (BCI) using prefrontal cortex activation due to mental arithmetic,” Scientific Reports, vol. 6, p. 36203, Nov. 2016.
     
  • A. Vrana, M. L. Meier, S. Hotz- Boendermaker, B. K. Humphreys, and F. Scholkmann, “Cortical Sensorimotor Processing of Painful Pressure in Patients with Chronic Lower Back Pain—An Optical Neuroimaging Study using fNIRS,” Front. Hum. Neurosci., vol. 10, 2016.
     
  • L.-C. Chen, M. Stropahl, M. Schönwiesner, and S. Debener, “Enhanced visual adaptation in cochlear implant users revealed by concurrent EEG-fNIRS,” Neuroimage, Sep. 2016.
     
  • L. Holper, E. Seifritz, and F. Scholkmann “Short-term pulse rate variability is better characterized by functional near- infrared spectroscopy than by photoplethysmography,” J Biomed Opt, vol. 21, no. 9, p. 91308, Sep. 2016.
     
  • H. O. Keles, R. L. Barbour, and A. Omurtag, “Hemodynamic correlates of spontaneous neural activity measured by human whole-head resting state EEG+fNIRS,” Neuroimage, vol. 138, pp. 76–87, Sep. 2016.
     
  • A. Vrana, M. L. Meier, S. Hotz- Boendermaker, B. K. Humphreys, and F. Scholkmann, “Different mechanosensory stimulations of the lower back elicit specific changes in hemodynamics and oxygenation in cortical sensorimotor areas—A fNIRS study,” Brain Behav, p. n/a- n/a, Sep. 2016.
     
  • I. Helmich, A. Berger, and H. Lausberg, “Neural Control of Posture in Individuals with Persisting Postconcussion Symptoms,” Med Sci Sports Exerc, Jul. 2016.
 

Don’t see your recent publication listed?

Have a publication coming out soon?

Please update us on your work!

We love to hear from our customers (especially when there is good news :D)! By keeping us informed on your latest publications, posters, presentations, and press/media (e.g., news articles, blog posts, videos, etc.) we are able to better serve both you and our community. We would be happy to consider highlighting you and your team on one of our upcoming newsletters, our social media page, or our website as well. Please send all research-related updates to support@nirx.net. Thank you for contributing to the NIRx community!

 

Recording Software Update: NIRStar (version 15-0)

The newest NIRStar version (15-0) is available for download! NIRx has worked hard to incorporate innovative and useful features.

The first, and by far most important, feature is the capacity to control fNIRS systems using a yet-to-be-released commercially probe (optode) prototype for 8mm short-distance measurements. (Stay tuned for the release date of this new probe!)


Second, we have implemented a Lab Streaming Layer protocol for real-time communication and synchronization with other systems (e.g. EEG, Eye- Tracking, etc.). 

Pictured Right: 

The Lab Streaming Layer (LSL) Extension Module  available in NIRStar 15-0 which will further enhance and enable custom real-time fNIRS processing protocols in multi-modal applications.


Third, further metrics to improve the data quality and its assessment, e.g. automatic cross- talk identification, are now available as well.

Pictured Left: NIRStar’s unique signal quality indicator (akin to EEG impedance checking).

 



Finally, there are also some key bug fixes and further usability enhancements to improve the interface and user experience. (A complete list of features, enhancements, and fixes are included in the accompanying user manual.)

Pictured right: NIRStar’s real- time block average view, which allows on-the-fly comparisons of Oxy-Hb and Deoxy-HB changes relative to incoming event markers.


We recommend all NIRx users to update their acquisition software ASAP and we are looking forward to your feedback. In the NIRx Help Center, you may report to us issues and desired features for next releases.

Please get in touch if you have questions related to switching from an older version of NIRStar to NIRStar 15-0.


Attention Customers: Register for NIRx’s
Dedicated fNIRS Technical Support Website

 

NIRx takes great pride in the quality of our technical and scientific support. We are researchers, physicists, and engineers and have gained great expertise working in fNIRS. We have created the NIRx Help Center to further engage with and assist our fNIRS customers.


Our aim is to maintain this as one of the best fNIRS learning resources available.  It offers a searchable knowledge base, a user forum, video tutorials, a download section and a support ticketing system.


A completely searchable knowledge base lets you search through the entire content by any keyword. The search engine will look for matching content in both the knowledge base and the user forum.


 

The videos section is an already large collection of all kinds of video tutorials: analysis tutorials, subject preparation and setup tutorials, videos from past NIRx workshops and so on.



 

The NIRx Help Center also offers a user forum to ask questions to the NIRx team or other members of the Help Center community.

You can find all kinds of downloads in the related section, including: the latest version of our software, user guides, quick start guides and tutorials, sample data, code samples and scripts, workshop talks, montages for NIRStar and more.

The best way to ask a support question for the NIRx team is to submit a ticket. This is the fastest way of reaching us for high priority issues.

You may access the Help Center from the Support section on our websiteThe NIRx Help Center is for NIRx users only, and registration is required. The majority of content is only available only upon password authentication. Please follow the register link in the top right corner of the home page. 

Register now! We are looking forward to seeing you in the Help Center!


fNIRS Detectors from NIRx - SiPD vs. APD

Overview

NIRx offers both ‘standard’ silicon photodiode (SiPD) and ‘high-sensitivity’ avalanche photodiode (APD) fNIRS
detectors. The NIRSport ‘Portable’ fNIRS System may only use the APD detectors with an additional coupling unit- the APDs in this case would not be for portable applications, and would furthermore require a different probe set from the standard probe sets generally. By contrast, the NIRScout ‘Lab-Based’ fNIRS System may use either the SiPD or APD detectors with identical probe sets.

 
 

fNIRS Detector SensitivitY: APD vs. SiPD Detectors

Our APD detectors are up to 8x as sensitive as our SiPD detectors on average (sensitivity differences vary with gain settings). This does give a much better signal overall, though detector sensitivity is not the only factor in determining signal level and quality.

 
 
 

Dynamic fNIRS Gain Settings

While considering detector sensitivity is critical, the system’s dynamic gain is also important when evaluating overall system performance. NIRx offers an industry- leading 9 levels of automated/programmable detector gain for both our SiPD and APD detectors. So, even though our SiPDs have less sensitivity than our APDs, we are able to make up for it against APD options offered by some of the other fNIRS manufacturers out there by maximizing the gain settings of the detectors.

 
 
 
 

Optimizing Unique fNIRS Detector Gain Settings

As part of every experiment, one must “calibrate” (AKA “optimize”) the exact gains used for every detector. Most systems only allow one gain setting per detector. By contrast, NIRx systems allow for unique gain settings for every source-detector pair. This is particularly important when a detector is positioned between two sources that are transmitting light through tissue with very different scattering and absorption properties. For example, imagine a detector placed on the hairline approximately around Fz in EEG standard coordinates, and this detector is measuring from two sources: source one is positioned 3cm anteriorly towards the nasion (~AFz in EEG coordinates) on a hairless part of the head with thinner scalp, skull and CSF, and source 2 positioned posteriorly towards the inion (~FCz in EEG coordinates) on a hairy part of the head with thicker scalp, skull and CSF. There would be substantially different amounts of scattering and absorption from each of these sources as the photons pass through the tissue, and thus a very different amount of light would actually reach the detector in between the two sources.   It would, therefore, be ideal for the detector to have a higher gain setting for source 2 (the source on the hair) vs. source 1 (the source on the forehead).

Unique detector gain settings for every source-detector pair are also important for multi-distance measurements and maximizing the number of usable data channels. This dynamic gain switching is the biggest advantage of the time- multiplexed recording mode used in NIRx devices compared to other ways to distinguish the signal from different sources.

 
 
 

Optimizing fNIRS Detector Gains and Signal

The process of setting gains differs substantially between systems. Some fNIRS systems even require the end-user to manually set each detector gain setting one- by-one for each measurement, which, as you can probably imagine, can take a very long time. NIRx’s recording software uses a user-friendly fully-automated signal optimization step which rapidly identifies the ideal signal level, and associated detector gains for each source-detector channel pairing.

 

 

Example of Unique Detector Gains Using NIRStar/NIRx Platform
1) Probe array (“Montage”):
An 8-source/8-detector (16-probe) montage is shown with 20x topographic data channels of interest positioned bilaterally over the motor cortex.
2) Montage Close Up:
Sources = S1, S2, S3, S4;
Detectors = D1, D2, D3, D4.
3) Topolayout/Signal Quality Indicator:
Shows 2-D blocked abstraction of montage, which allows easy-to-view color-based/changing signal quality and levels. Each number pair corresponds to a respective source and detector number, in that order, for that data channel of interest (i.e., “2-1” = channel formed by source #1 and detector #2). Note: signal quality has already been calibrated/optimized at this point and that the source-detector pairs seen in the montage close up
4) Gain Settings Map:
Shows the detector gain level for each source-detector pair. Note channels “2-1” and ”1-1” in particular for the moment. Detector #1 is part of both channels but has a substantially different gain setting for each channel. Channel 2-1 has a gain of 3, whereas channel 1-1 has a gain of 5. This dynamic gain switching for a single detector with multiple sources greatly improves ease of use and signal quality.
 

Detector Probes and Caps

 
Fiber Optic Detector.JPG

Achieving optimal signal quality involves more than just the system electronics and control software. The design and fit of the physical detector hardware, and its connecting components, are arguably just as important. Imagine a car with no or very poor tires - it won’t get you very far, will it? Well, probes and caps are really just like the tires for your fNIRS system. Without them, you would not be able to collect any data, despite how powerful the system’s engine may be. We have put extensive research and user-feedback into our probe and cap design. While we could tell you a lot more about it here, this is really better seen than explained. You can see here just how fast and easy our cap setup is. And  you can learn more about the probes and caps on our website.

 
 

Which Detector to Choose: APD or SiPD?

It may come as no surprise, but the APD detectors are quite a bit more expensive than the SiPDs. That said, their value is unquestionable: a NIRx APD detector system, along with our innovative probe systems, will work on the vast majority of subjects, measuring from any part of the head, with an incredibly easy/fast setup.

Our APDs also are best for end-users that wish to do fMRI/fNIRS or fMRI/MEG studies.

The SiPD detectors will work excellent on child and geriatric subjects on any part of the head, and will work with ~60-70% of college-aged subjects (FYI: college students are generally some of the most difficult subjects due to their full-grown heads and thicker hair) with thick  black hair (thicker,  darker hair is more difficult than thinner, lighter hair) during measurements on top of their head (e.g., the motor, somatosensory cortex, etc.). In total it generally averages out to ~80% of subjects in multi-cultural locations, such as big cities in USA, Canada, UK, Germany, France, etc..

All that said, this still could be a major factor for you. It is important to remember: with NIRx systems you can start with SiPD detectors and switch over to APD detectors. We offer one of the most versatile fNIRS platforms out there in terms of upgrade options and flexibility. Please do let us know what your decision context is and we will be happy to work with you to provide you the best-possible solution.


Laser and LED fNIRS Sources from NIRx
 

NIRx’s History with Laser & LED sources

As you may know, NIRx started out of the lab of Dr. Randall Barbour at SUNY Downstate Medical Center in the mid-1980s. It wasn’t until 2000 that NIRx formed and sold its first NIRS system, the “DYNOT” (Dynamic Optical Tomography); this laser-based system used straight fiber optic probes which could be oriented in very high-density grids for tomographic imaging. In fact, all of Dr. Barbour and NIRx’s systems were laser based from the 1980s onward, until 2010, when the first “NIRScout” system was released. As many of you know, this first-generation NIRScout used LED instead of Laser sources for light illumination.

In 2015, NIRx introduced Lasers to the NIRScout family. The new “NIRScoutX+” chassis enabled the breakthrough of the first hybrid LED/Laser system. Users can now choose either LED or Laser sources with this system, enabling for the ideal source choice by application.

 
 

A Comparison of Laser vs. LED sources:

Fiber Optic Performance

Lasers are a better option than LEDs for NIRS measurements that require fiber optics, like those done with MRI and MEG. The collimated-bandwidth Laser light couples much more efficiently into a fiber cable than the LED light. For MRI and MEG-concurrent NIRS studies, in particular, very long probes are necessary as the NIRS system sits in the control room, and its probes (usually ~8-10m long) go to and from the subject’s head in the scanning room.

 

  • Fiber Optic Performance Summary

    • Lasers beat out LEDs in applications where fiber optic probes are necessary (e.g., NIRS/fMRI, NIRS/MEG, collocated NIRS-TMS, etc.)
 
 
 

Cable Length, Weight, and Flexibility

It is also important to note that cable length is a factor for overall signal levels. Light signal, whether from Lasers or LEDs, attenuates as it passes through fiber optic cables. The level of attenuation does vary based on the properties of the particular fiber, though shorter fibers will always outperform longer fibers of the same make/type, assuming all else equal.

NIRx does recommend our very high-powered active LEDs for most use cases (we will cover why that is later).

The  two main  differences between our active and  fiber optic sources:
 
1)  The location of the NIRS source
      a.  Active NIRS sources are contained within the probe tip housing
      b.  Fiber optic NIRS sources are contained within the NIRS system itself
 
2)  The material of the probe cables
      a.  Active sources  use   electronic  cables:  very lightweight, durable, and may be extended without significant signal loss (i.e., you may have one  set of probes with multiple extensions if you like)
      b.  Fiber optic sources use glass cables: relatively heavier; flexible, but less durable; may not be extended (i.e., you need multiple probe sets if different cable lengths are required)
 

The location of the active LED in the probe tip housing minimizes signal attenuation, which greatly improves overall system performance. The lighter weight cabling with active LEDs is very helpful in child and mobile applications, though all subjects appreciate less weight on their heads during measurements.
 

  • Cable Length, Weight, and Flexibility Summary

    • NIRx Active LEDs have far less cable-length- related attenuation than fiber optic lasers
    • NIRx Active LEDs are much lighter than fiber optic lasers
    • NIRx Active LEDs are more flexible than lasers
    • Note: as mentioned above, Lasers beat out LEDs when fiber optic probes are necessary (e.g., NIRS/fMRI, NIRS/MEG applications, etc.)
 
 
 

Multi-Wavelength Considerations

Most NIRS system use 2 wavelengths to distinguish between oxy- and deoxy-hemoglobin. NIRx LED systems are only offered with 2 wavelengths, though, as mentioned, one may own a hybrid system with both LEDs and Lasers. By contrast, Lasers may have many more than 2 wavelengths (but typically also have just 2 wavelengths in most commercial systems). NIRx systems currently offer 2, 4 and 8-wavelength source options.

Higher wavelength counts yield a potentially better characterization of the Hb signal (though, how much better is under debate) as each wavelength has a slightly different depth of penetration, differential pathlength factor (dpf), and associated molar extinction coefficient for the respective chromophores of interest: generally, oxy-Hb and deoxy-Hb. Note: some researchers are interested in using NIRS to identify cytochrome c-oxidase (see fig. 1 right), though this methodology is still being refined.

That said, the information gained from the additional wavelengths does not have a clear-cut advantage at the moment for researchers looking at only oxy- and deoxy-hemoglobin. And when considering the great increase in cost one must pay for a system with more wavelengths, it may not be worth it for you to upgrade to more wavelengths.
 

 

Multi-Wavelength Considerations Summary

  • NIRx LEDs are 2-wavelength sources
  • NIRx  Lasers may be 2 or 4-wavelength sources (note: 8-wavlength custom option available)
  • Additional wavelengths come at a great price
  • The information gained from additional wavelengths may not be worth the cost of the upgrade if all you are interested in is hemoglobin changes
 
 

Ideal Applications for NIRx LEDs and Lasers

NIRx LEDs may be used in nearly any application, even MRI and MEG-concurrent measurements, though those particular applications are best left to Laser sources. LEDs are generally better when considering child and mobile applications, as they are lighter and more flexible than Lasers. We recommend you use Laser sources if you have used them before and your research requires it, or if you want to do MRI or MEG-concurrent measurements.

 
 

Conclusion - Not all LEDs (or Lasers) are created Equal

As you might imagine, the finer points of producing a source do greatly matter. While there are many LED and Laser systems out there, please do keep in mind that NIRx has extensively tested the components used in its systems, and that we validate the use of our different source options before they become commercially-available. Our customers have made us aware of other manufacturers that offer LEDs which  do not offer a signal strength sufficient enough to be effectively used on top of an adult’s head who has thick, black hair. We have even  heard of Laser systems which have this issue as well. Actually, while source type and strength is an important factor in how one may apply NIRS, there are many other considerations, including: probe shape, grommet type, NIRScap shape and type, and even software/system control issues. Needless to say, we have considered these many factors (and more) in our developments; this becomes apparent when comparing different NIRS systems first-hand.

All that said, the most-important factor for any NIRS source is photon output: how much light does the source actually put out at its tip (and into the skin when applied to a subject) for measurements? This is best measured with phantoms and equivalent light detectors. In our tests we have found that our LEDs and Lasers have the same output at the probe tip.

NIRx’s LED and Laser systems are both very well published and an excellent option for  NIRS researchers. You can see a sample of the publications done on our website here: www.nirx.net/publications. If you’d like to learn more about our systems, please get in touch: www.nirx.net/contact.
 

Overall Summary

  • Source type matters less than light output and application requirements
  • Just because a system looks good on paper does not mean it will work for you in your reseach
    • Specs do not always equal success!
  • LEDs and Lasers really are not that different in the end, it all depends on the photon output and your practical needs
  • Check out systems before you make your final purchase decision:
    • Some NIRS systems may not offer the performance you require for your application
    • NIRx  offers  quick  online  demonstrations with relatively short notice


[2]   A Bakker et al. “Near-Infrared Spectroscopy.” Chapter. April 2012

 

 ‘Like’ and Follow us on Facebook:

 
 
 
square logo with ON.jpg
 

© 2018 by NIRx Medical Technologies. All rights reserved.